Rotor Selection Guide

Alfa Wassermann offers a broad range of rotors each uniquely suited to specific particle applications and desired volumes. Rotor volumes range from as little as 0.19 L to 8.4 L for batch or continuous flow operations. Common applications include Influenza, AAV, Adenovirus, Hepatitis B, Rabies, and more.

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The Alfa Wassermann Advantage

Linear Scale Rotor Cores

The linear scalable ultracentrifuge rotors from Alfa Wassermann are unique among ultracentrifuges. They are designed with scalable applications in mind to allow the user to generate the same separation profile at different process volumes when using AWST ultracentrifuge systems. Having this capability to go from research to pilot scale to full scale production results in enormous cost savings. This patented linear scale core technology enables a direct scale up of separation between the research scale Promatix 1000™ to the PKII and to the KII manufacturing scale Ultracentrifuge.

Once a series of separation parameters (such as volume, path length, centrifugal force and run time) have been proven to work the process can be fully scaled up or down.  There is no need for further alterations or extensive testing and re-validation, which results in significant cost savings. A 14 fold scale down is achievable through this cost effective and labor saving approach.

Each rotor assembly is composed of a tubular bowl and two end caps which are made of titanium alloy. Contained within the titanium bowl is the Noryl® rotor core. This assembly forms flow channels down which process material flows during operation.

Maximum Speed and Centrifugal Force 40,500 rpm, 121,000 xg
Flow Rate Range 0 L/h (batch) up to 60 L/h
Process Volume 500 mL to 200 L continuous fl ow
Rotor Type Application Max. Force Capacity with Core Dimensions
K3 PKII PK3
K3 PKII PK3
Continuous flow Isopycnic banding At 40,500 rpm
Rmax: 121,200 xg
Rmin: 100,000 xg
K factor: 29.7
3.2 L
1.6 L
0.8 L
0.4 L
Diameter:
Max: 130 mm
Min: 110 mm
Path Length: 11 mm
K5
K5
Rate zonal separations At 40,500 rpm
Rmax: 121,200 xg
Rmin: 38,500 xg
K factor: 177
8.4 L Diameter:
Max: 130 mm
Min: 42 mm
Path Length: 45 mm
K5
K5
Continuous flow isopycnic banding with pre-clarifier At 40,500 rpm
Rmax: 121,200 xg
Rmin: 100,000 xg
K factor: 29.7

Pre-clarifier:
Rmax: 53,900 xg
Rmin: 49,000 xg
3.2 L

Pre-clarifier: 0.17 L
Diameter:
Max: 130 mm
Min: 110 mm
Path Length: 11 mm

Pre-clarifier Diameter:
Rmax: 58 mm
Rmin: 23 mm
K10 PK10
K10 PK10
Isopycnic banding separation At 40,500 rpm
Rmax: 121,200 xg
Rmin: 38,500 xg
K factor: 140
8.0 L
4.0 L
Diameter:
Max: 130 mm
Min: 53 mm
Path Length: 39 mm

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Our Mission

To be the supplier whose singular purpose is the support of bioprocess and pharmaceutical industries by providing expertise in continuous flow UCF separation applications and now fluid handling solutions.

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USA

Alfa Wassermann Inc . 4 Henderson Drive
West Caldwell . NJ 07006 . USA

Telephone +1 800 220 4488
Fax +1 973 276 0383


Netherlands

Alfa Wassermann B.V. Lorentzlaan 5
3401 MX IJsselstein . Netherlands

Telephone +31 348 487 300
Fax +31 348 433 000

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