Reference Library-Technical Note

The Reorienting Gradient Technique

The density gradient is loaded into the rotor while it is at rest (1). As the rotor is gradually accelerated, the gradient reorients itself vertically along the wall (2). Sample fluid is now pumped into the rotor at one end on a continuous flow basis (3).
The sample particles sediment radially into the gradient of increasing density. They eventually band (iso-pycnically) in cylindrical zones where the gradient density equals a particle’s buoyant density (4).
At the end of the run, the rotor is decelerated (5) and the gradient reorients itself to the original position without disturbing the particle bands (6).
The banded particles are now ready to be unloaded when the rotor is at rest (7). Fractions are collected using a small peristaltic pump or air pressure.

Downloads

Printable Versions:

Technical Notes

AlfaSIP™ - Sanitization by Pure Chlorine Dioxide

Custom Installation

Gradient Materials

Linear Scale-up Rotors for KII & PKII Ultracentrifuges

Protocol Development Technology on the AW Promatix 1000™

The Reorienting Gradient Technique

Single Step Clarification, Concentration and Purification

Application Notes

K Series Rotors for KII Production Scale Ultracentrifuges

PK Series Rotors for PKII Pilot Scale Ultracentrifuges

Return to Tech/App Note list