Reference Library-Technical Note

Protocol Development Technology on the AW Promatix 1000™ BioPurification System

AW Promatix 1000™

Versatility
A versatile BioPurification system, offering fully automated and hands-free sample purification; including
  -   Gradient formation
  -   Sample loading
  -   Separation
  -   Isolation
  -   Concentration
  -   Gradient fraction collection
  -   System cleaning (before and/or after the run cycle)

AW Promatix 1000™
Innovation
This unique BioPurification system, also offers
High performance purification
   based on AW's patented and industry proven
   ultracentrifugation technology
Increased efficiency in laboratory testing,
   simple operation and walk-away automation Windows based operating platform,
   user programmable control system and application menu

AW Promatix 1000™
Your Purification Partner for...
Automatic, reliable and reproducible sample purification
  -   User defined pre-selectable protocols, for reproducible
       operation
  -   Start to finish temperature control, for optimized sample
       integrity
Development and optimization of purification methodologies
Product isolation and characterization
Quality Control testing

AW Promatix 1000™
Wide Range of Applications
Purification of bio molecules, ranging from cellular organelles to large molecules, including:
  -   Viral and non-viral vectors, for gene therapy products and
       therapeutic vaccines
  -   Virus products, for therapeutic and prophylactic vaccines
  -   Bacterial subcomponents, for prophylactic vaccines
  -   Cellular subcomponents, for isolation of DNA, RNA,
      organelles
  -   Macromolecules

AW Promatix 1000™
Superior Functionality
Sample size ranging from 5 mL to 10 Liters
  -   Range of scaleable rotors (AW patented technology) with
      different separation chamber volumes available
  -   User selected batch or continuous flow processing
R&D protocols directly transferable to Production
  -   Easy upscale to Alfa Wassermann Pilot Scale PKII and Full
      Production Scale KII Ultracentrifuges
  -   Rotor characteristics identical to PKII and KII production
      rotors —Same separation results!
  -   No up-scaling trouble shooting, accelerate time to market
  -   Save time and cost

AW Promatix 1000™
Sample Integrity Protected
Optimal sample protection
  -   All solutions including sample and final fractions remain
      in a temperature controlled environment from start to
      finish
  -   Temperature maintained between 4 and 10 ºC on whole
       fluid system, including fraction collector for 100 mL rotor
Automated fraction collection
  -   Fractions collected in optional deepwell microplate
      fraction collector (for 100 mL rotor)
  -   Robotic sample handling or analyzer systems can be
      interfaced
  -   External fraction collector can be provided for larger
      fractions

AW Promatix 1000™
Reproducibility through Automation
Highly reproducible results
  -   User selected protocols, recalled from hard drive
  -   Protocols are pre-programmed by user
       -   Repeated runs produce identical characteristics
  -   Density gradient is prepared automatically and precisely
  -   Buffer and sample feed are automated
  -   Sample fraction collection is automated

AW Promatix 1000™
Safe & Easy Operation
Easy to use
  -   Guidance through the set up and operation
  -   Automated, hands-free running
  -   Automated cleaning and sanitizing
       -   Programmable to run before and after separation cycle
  -   Easy exchange of fluid lines and valves, replaceable in
      minutes
Safe for sample and user
  -   Self Diagnostics system, unattended operation
  -   On-board alarm system with color coded alerts
  -   28 point monitoring of all functions

AW Promatix 1000™
Reporting Your Way
Versatile reporting of data, in Universal data format (.cvs)
  -   LIMS compatible for integrated reporting
  -   Windows format uses your favorite database or
      spreadsheet
  -   All types of graphics, tables and charts available
  -   Run overlays can show difference in operation
No dedicated or complex reporting software to learn
  -   Be operational immediately!

AW Promatix 1000™
Even More Benefits!
Ease of Installation
  -   Small footprint (128 x 83 cm; 50 x 33 inch)
  -   Simple to install or reposition (no bolting to the floor)
  -   Operates on electricity only, 230V, 50 or 60 Hz, 35A
And in the future...
  -   Production capabilities for high yield, high value molecules

AW Promatix 1000™
Comparison to Lab Ultracentrifuges
Lab Ultracentrifuges are aimed at laboratory testing
   of several samples in 1 run, or small volumes
AW Promatix 1000™ is aimed at Industry R&D and
   protocol development for production
  -   Full automation, from sample feed to fractions collected
  -   Optimal sample protection by 100% temperature control
  -   Batch or continuous flow processing, from mL to Liters
  -   Automated reproducible gradient formation and
      sanitization
  -   Identical rotor geometry to production centrifuges
  -   Volume scale-up across a range of rotors, AW patented
      technology
  -   Batch data stored on hard drive, run-to-run comparison
      reports
  -   Versatile data handling, Windows and LIMS compliant
  -   and more...

AW Promatix 1000™ BioPurification System
      From R&D to Production in One Easy Step

AW Promatix 1000™ Rental Opportunity

Downloads

Printable Versions:

Technical Notes

AlfaSIP™ - Sanitization by Pure Chlorine Dioxide

Custom Installation

Gradient Materials

Linear Scale-up Rotors for KII & PKII Ultracentrifuges

Protocol Development Technology on the AW Promatix 1000™

The Reorienting Gradient Technique

Single Step Clarification, Concentration and Purification

Application Notes

K Series Rotors for KII Production Scale Ultracentrifuges

PK Series Rotors for PKII Pilot Scale Ultracentrifuges

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