Reference Library-Technical Note

Linear Scale-up Rotors for KII & PKII Ultracentrifuges

Patented Linear Scale-up Rotors™ from Alfa Wassermann Separation Technologies eliminate cost- and time-consuming redevelopment of protocols when moving from final research volumes, through pilot scale to production levels.

Separation occurs in the path length from the core to the outer edge of the rotor. Fluid enters the rotor and moves along the core. Particles travel the effective path length from minimum radius (core) to achieve their static level within the radius of centrifugation, the maximum radius of the rotor being the maximum possible and only achieved during pelleting.

Most brands of centrifuge use rotors of different volume and radius when different volumes of fluid are to be treated. The different geometry creates varied effects and direct transfer of a protocol is not possible.

AWST Linear Scale-up Rotors™ provide the answer. The distance from the surface of the core (the minimum radius) to the inner surface of the rotor body (the maximum radius) remains identical. For small volumes, the fins propelling the fluid in rotation are wide; as rotor volumes increase, the fins of the cores are narrower, leaving a wider flowpath.

When developing a protocol and starting with minimum volume on an ePKII or PKII Pilot Scale Ultracentrifuge with PK3-200 rotor, changing to maximum volume on an eKII or KII Production Scale Ultracentrifuge with a K3 rotor gives a linear scale-up factor of 16:1 or more depending on actual volumes treated. The use of an AW Promatix 1000 BioPurification System with a PX3 rotor can boost this ratio even more, permitting dramatic scale-up from a protocol developed in R&D to full production overnight.

Exactly the same time will be used to process 16x as much fluid as for the original volume.
No separation parameter is changed.
No protocol development is required.
Large time savings are achieved with guaranteed results.

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Technical Notes

AlfaSIP™ - Sanitization by Pure Chlorine Dioxide

Custom Installation

Gradient Materials

Linear Scale-up Rotors for KII & PKII Ultracentrifuges

Protocol Development Technology on the AW Promatix 1000™

The Reorienting Gradient Technique

Single Step Clarification, Concentration and Purification

Application Notes

K Series Rotors for KII Production Scale Ultracentrifuges

PK Series Rotors for PKII Pilot Scale Ultracentrifuges

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