Reference Library-Application Note

Downloads

PK Series Rotors for PKII Pilot Scale Ultracentrifuges

Printable Versions:

Applications

Designed to take separations from final research to pilot scale processing, PK Series Rotors are used for particle separation via rate zonal separation and isopycnic banding, and can accept corrosive gradients.

Particle types separated include viruses, ribosomes, and other subcellular units with human and animal vaccines, viral vectors, gene therapy, cancer studies, serum and blood fractionation among the important fields of application.

Particles are separated on the basis of their buoyant density. They move under the force of gravity to the band with the same density as their own and remain in this band until recovered by discharging the rotor in liquid fractions after rotation stops.

These rotors can also be used for simple pelleting where the particles are recovered at the end of the run after adhering to the wall of the rotor.

PK3 models provide linear scale-up to simplify protocol development for increasing sample volumes.

The PK6 includes a Pre-Clarifier to eliminate the need for a separate step, saving time and increasing convenience and avoiding any possible contamination due to additional sample handling.

Specifications

Rotor Body – All PK Series Rotors

Max.
RCF
(x g)

Max.
Speed
(rpm)

Max.
Radius
(mm)

Residence Length
(cm)

Materials

121,030

40,500

Titanium

Rotor Cores

Materials – Rotor cores can be in Noryl^® (most frequent choice) PEEK™ or Titanium

Type

Min. RCF
(x g) at Max. rpm

Min
Radius (mm)

Path
Length (mm)

Volume (Liters)

PK3*

99,940

54.5

11.5

1.6

PK3-800*

99,940

54.5

11.5

0.8

PK3-400*

99,940

54.5

11.5

0.4

PK3-200*

99,940

54.5

11.5

0.2

PK6
.
Built-in Pre-
Clarifier

PK10

48,960

26.7

39.3

4.0

PK11

120,110

65.5

0.5

0.19

*After the purchase of one PK3 series rotor, other cores can be fitted to change the volume.

Linear scale-up
When developing a protocol and starting with a PKII Pilot Scale Centrifuge with PK3-200 rotor, changing to a PK3 rotor with standard 1.6 liter core gives a linear scale-up factor of 8:1.

Exactly the same time will be used to process 8x as much fluid as for the original volume.
No separation parameter is changed. No protocol development is required.
Massive time savings are achieved with guaranteed results.

Changing to a KII Production Scale Ultracentrifuge with K3 rotor gives a 16:1 scale-up factor with the same advantages.

Max. RCF
53,180

Max. Rad
29

Technical Notes

AlfaSIP™ - Sanitization by Pure Chlorine Dioxide

Custom Installation

Gradient Materials

Linear Scale-up Rotors for KII & PKII Ultracentrifuges

Protocol Development Technology on the AW Promatix 1000™

The Reorienting Gradient Technique

Single Step Clarification, Concentration and Purification

Application Notes

K Series Rotors for KII Production Scale Ultracentrifuges

PK Series Rotors for PKII Pilot Scale Ultracentrifuges

Return to Tech/App Note list