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K Series Rotors for KII Production Scale Ultracentrifuges

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Applications

Specifications

Responding to production needs for the pharmaceutical industry and now found increasingly in biotechnology, K Series Rotors are used for particle separation via rate zonal separation and isopycnic banding, and can accept corrosive gradients.

Particle types separated include viruses, ribosomes, and other subcellular units with human and animal vaccines, viral vectors, gene therapy, cancer studies, serum and blood fractionation among the important fields of application.

Particles are separated on the basis of their buoyant density. They move under the force of gravity to the band with the same density as their own and remain in this band until recovered by discharging the rotor in liquid fractions after rotation stops.

These rotors can also be used for simple pelleting where the particles are recovered at the end of the run after adhering to the wall of the rotor.

The K3 model completes the linear scale-up range that commenced with the PK3 series of the PKII Pilot Scale UltraCentrifuge. This simplifies protocol development for increasing sample volumes.

The K6 includes a Pre-Clarifier to eliminate the need for a separate step, saving time and increasing convenience and avoiding any possible contamination due to additional sample handling.

Rotor Body – All K Series Rotors

Max.
RCF
(x g)

Max.
Speed
(rpm)

Max.
Radius
(mm)

Residence Length
(cm)

Materials

121,030

40,500

Titanium

Rotor Cores

Materials – Rotor cores can be in Noryl^® (most frequent choice) PEEK™ or Titanium

Type

Min. RCF
(x g) at
Max. rpm

Min
Radius (mm)

Path
Length (mm)

Volume (Liters)

99,940

54.5

11.5

3.2

K5 (batch rotor)

38,510

8.4

K6
.
Built-in
Pre-
Clarifier

K10

48,960

26.7

39.3

8.0

K11

120,110

65.5

0.5

0.38

Linear scale-up
When developing a protocol and starting with a PKII Pilot Scale Centrifuge with PK3-200 rotor, changing to a KII Production Scale UltraCentrifuge gives a linear scale-up factor of 16:1.

Exactly the same time will be used to process 16x as much fluid as for the original volume.
No separation parameter is changed. No protocol development is required.
Massive time savings are achieved with guaranteed results.

Max. RCF
53,180

Max. Rad
29

Technical Notes

AlfaSIP™ - Sanitization by Pure Chlorine Dioxide

Custom Installation

Gradient Materials

Linear Scale-up Rotors for KII & PKII Ultracentrifuges

Protocol Development Technology on the AW Promatix 1000™

The Reorienting Gradient Technique

Single Step Clarification, Concentration and Purification

Application Notes

K Series Rotors for KII Production Scale Ultracentrifuges

PK Series Rotors for PKII Pilot Scale Ultracentrifuges

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