Products

AW Promatix 1000™ - BioPurification System for R&D: Ultracentrifugation Technology with Fraction Collector

The AW Promatix 1000™ from Alfa Wasserman is designed to develop methodologies for the separation, fractionation and concentration of viral vectors, virus-like particles, viruses, viral and bacterial vaccines, macromolecules and cellular organelles. The strength of the system is its versatility; with full programmability for successful separation protocols, automated fraction collection option to facilitate analysis and linear scale-up to pilot scale and full production.

Purification protocol optimization - higher recovery & purity
     Most run parameters can be modified; temperature
     regulated environments
Simple Scale-up of R&D methods to production
     Sample quantity from 50 mL to several liters in R&D
Complete BioPurification System
     Combines ultracentrifugation technology, gradient
     production device, fraction collection and control system
Quality Control - sample characterization profiles
Easy to use and full auto-diagnostics
Automated sanitization cycle - no contamination
Safety for sample & user - Multi-point monitoring

Now AWST offers a full range of ultracentrifuges operating from a few mL to hundreds of liters. No need to develop protocols on a laboratory ultracentrifuge of a different geometry and then labor to transpose the parameters through approximate calculations to a production instrument. All the knowledge gained from the AW Promatix 1000 remains usable with larger capacities. This eliminates a large part of development work, enabling you to get your products to market in a shorter time and reap the rewards of higher margins and reference positioning.

FAQs about the AW Promatix 1000™

How does scale-up work between AW Promatix 1000 and PKII pilot scale or KII production scale ultracentrifuges?
The PX3 series of rotors for the AW Promatix 1000 has exactly the same radius inside the body of the rotor as the PK3 series for PKII and the K3 series for KII machines. The radius of the base of the channel of the rotor core is also identical across all 3 series. Thus the path traveled by the particle through the density gradient (from the base of the channel to the radius of buoyant density) remains the same, whatever the rotor.

The channel length is twice as long in the PKII and four times as long in the KII for an otherwise identical rotor assembly. The available volume for particle concentration is increased by the same proportions. This is what permits scale-up. Processing flow rate is faster in the longer rotors as the residence time inside the rotor only needs to be the same as in shorter rotors.

How is the scale of a few mL to hundreds of liters achieved if the rotor volume ratios are only two or four to one?
Each ultracentrifuge has its own range of rotor cores. The minimum volume of sample fluid is processed in a rotor with a core containing narrow channels. The maximum volume is processed with a core of very wide channels. The separation path length remains the same in all cores and rotors of the series "3". Also, sample concentration can be stopped at any time, not only when the product density band is full, so the volumes treated by one rotor assembly are widely variable.

What thermal protection is there for the sample?
Sample, buffer, density gradient components and fluids for recycling are all held in chilled reservoirs. The rotor chamber is also chilled, as is the fraction collector chamber. Temperature is maintained within the range of 4-10 °C.

How can I obtain good repeatability?
The AW Promatix 1000 is fully automated for the operating cycle. A virtually unlimited set of protocol parameters can be stored. Once recalled the automation ensures that conditions are reproduced precisely.

Density gradients can be difficult to prepare. How is this done in the AW Promatix 1000?
The AW Promatix 1000 offers fully automatic creation of density gradients. It suffices to fill the reservoirs with the components, select the suitable program and the AW Promatix 1000 takes care of the rest.

What format is data stored in on the AW Promatix 1000?
Data is stored in the format that can be read by all databases, spreadsheets and LIMS. This is .csv (comma separated values) also known as comma delimited. An external system can search for data on the hard drive or data can be sent to an external drive. Once read in its basic .csv format, this data can then be transformed by the user's software into whatever format he prefers.

What reports can be produced from the AW Promatix 1000?
The AW Promatix 1000 data is most usually rendered by a spreadsheet such as Excel. It is thus possible to choose from the huge selection of available reporting and data representations supplied with such programs. These included data tables, Speed vs Time graphs and run comparison with multiple overlays.

How easy is it to learn to use the AW Promatix 1000?
A step-by-step on-screen guide leads you through each part of the operation. The AW Promatix 1000 is one of the easiest to use automated instruments that you will have in your lab. Our system is designed to free up your valuable time, enabling you to get on with other important tasks, while the AW Promatix 1000 takes care of complex operations.

What do I do if something goes wrong on the AW Promatix 1000?
Built into the control system is an auto-diagnostic feature. The AW Promatix 1000 will indicate not only the source of the error but will display the possible causes of the problem so you can soon be back to normal operation.

How can I be sure that my sample is being treated correctly in the AW Promatix 1000?
The AW Promatix 1000 incorporates around 28 monitoring points covering all aspects of its operation. All alarm events are displayed on screen as a descriptive table, together with the time that they were triggered and when they were cleared.

What are the installation requirements for the AW Promatix 1000?
The AW Promatix 1000 only requires electrical service utilities. A 230V and 50 or 60Hz single phase line fused at 35A is all that is needed. The footprint of the ultracentrifuge is 127.8 cm (50.3 in) width by 83.1 cm (32.7 in) deep and height is 172.7 cm (68.0 in). Installed footprint including access space is recommended to be 188.7 cm (74.3 in) wide, 190.5 cm (75.0 in) deep and 182.9 cm (72.0 in) high.

AW Promatix 1000™

AW Promatix 1000™ Rental Opportunity

Download your copy of the AW Promatix 1000™ flyer

Come and meet us at one of several events to discuss what the Promatix can do for you.

More technical information? The Reference Library has what you need for Promatix and many other products.